Search Results: crispr-cas-a-laboratory-manual

Crispr-Cas: A Laboratory Manual

Author: Jennifer Doudna,Prashant Mali

Publisher: N.A

ISBN: 9781621821311

Category: Medical

Page: 182

View: 1977

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign DNA, these powerful techniques allow investigators to edit DNA sequences and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system-especially for minimizing off-target effects-are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.

CRISPR-Cas

Author: University Jennifer Doudna,Prashant Mali

Publisher: N.A

ISBN: 9781621821304

Category: RNA

Page: 192

View: 9886

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.

RNA

A Laboratory Manual

Author: Donald Charles Rio

Publisher: N.A

ISBN: 9780879698904

Category: Science

Page: 586

View: 4824

Almost all molecular and cellular biology laboratories now handle RNA and this manual is an authoritative source of information and protocols for this purpose, from the basic to the advanced. Required reading for every research laboratory in the life sciences.

The Condensed Protocols from Molecular Cloning : a Laboratory Manual

Author: Joseph Sambrook,David William Russell

Publisher: CSHL Press

ISBN: 0879697717

Category: Science

Page: 800

View: 5236

The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.

Mouse Hematology

A Laboratory Manual

Author: Michael P. McGarry,Cheryl A. Protheroe,James J. Lee

Publisher: N.A

ISBN: 9780879698850

Category: Science

Page: 99

View: 7340

Mice are used extensively in studies of blood, the immune system, and inflammation. This book is a concise review of conventional methods for preparing and examining blood and blood-forming tissues. It contains a short laboratory manual featuring detailed protocols, a DVD of video demonstrations, and a poster of blood cell types seen microscopically.

Proteomics in Practice

A Guide to Successful Experimental Design

Author: Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker

Publisher: John Wiley & Sons

ISBN: 3527622306

Category: Science

Page: 502

View: 2441

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

Modern Prometheus

Author: Jim Kozubek

Publisher: Cambridge University Press

ISBN: 1108454623

Category: Law

Page: 478

View: 7143

This book tells the dramatic story of Crispr and the potential impact of this gene-editing technology.

Live Cell Imaging

A Laboratory Manual

Author: Robert D. Goldman,David L. Spector

Publisher: CSHL Press

ISBN: 9780879696832

Category: Science

Page: 631

View: 4598

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

CRISPR

Methods and Protocols

Author: Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fineran

Publisher: Humana Press

ISBN: 9781493926862

Category: Medical

Page: 366

View: 7645

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

Genome Editing and Engineering

From TALENs, ZFNs and CRISPRs to Molecular Surgery

Author: Krishnarao Appasani

Publisher: Cambridge University Press

ISBN: 1107170370

Category: Science

Page: 586

View: 9747

A complete guide to endonuclease-based genomic engineering, from basic science to application in disease biology and clinical treatment.

PCR primer

a laboratory manual

Author: Carl W. Dieffenbach,Gabriela S. Dveksler

Publisher: Cold Spring Harbor Laboratory Pr

ISBN: N.A

Category: Business & Economics

Page: 520

View: 3350

This second edition of a much-praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.

A Crack in Creation

Gene Editing and the Unthinkable Power to Control Evolution

Author: Jennifer A. Doudna,Samuel H. Sternberg

Publisher: Houghton Mifflin Harcourt

ISBN: 0544716965

Category: Science

Page: 320

View: 5986

Finalist for the Los Angeles Times Book Prize One of Science News’ Favorite Books of the Year “Required reading for every concerned citizen.” — New York Review of Books “The future is in our hands as never before, and this book explains the stakes like no other.” — George Lucas Not since the atomic bomb has a technology so alarmed its inventors that they warned the world about its use. That is, until 2015, when biologist Jennifer Doudna called for a worldwide moratorium on the use of the gene-editing tool CRISPR—a revolutionary new technology that she helped create—to make heritable changes in human embryos. The cheapest, simplest, most effective way of manipulating DNA ever known, CRISPR may well give us the cure to HIV, genetic diseases, and some cancers. Yet even the tiniest changes to DNA could have myriad, unforeseeable consequences—to say nothing of the ethical and societal repercussions of intentionally mutating embryos to create “better” humans. Writing with fellow researcher Sam Sternberg, Doudna shares the thrilling story of her discovery and describes the enormous responsibility that comes with the power to rewrite the code of life. “An essential start to educating the public . . . reveal[s] the complex, interlocking, and thoroughly international nature of today’s bioscience.” —Los Angeles Review of Books “An invaluable account . . . We owe Doudna several times over.” — Guardian

Antibodies

A Laboratory Manual

Author: Edward A. Greenfield

Publisher: Anchor Books

ISBN: 9781936113804

Category: Science

Page: 847

View: 1424

"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"--

Methods in plant molecular biology

a laboratory course manual

Author: P. Maliga

Publisher: Cold Spring Harbor Laboratory Pr

ISBN: N.A

Category: Science

Page: 446

View: 7294

Purifying Proteins for Proteomics

A Laboratory Manual

Author: Richard J. Simpson

Publisher: N.A

ISBN: 9780879696955

Category: Science

Page: 801

View: 7486

This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.

Crispr Technology

The Revolutionary Breakthrough for Genetics & Evolution

Author: Paul F. Kisak

Publisher: Createspace Independent Publishing Platform

ISBN: 9781548451516

Category:

Page: 478

View: 5629

CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeats which are segments of prokaryotic DNA containing short, repetitive base sequences. These play a key role in a bacterial defense system and form the basis of a genome editing technology known as CRISPR/Cas9 that allows permanent modification of genes within organisms. In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated system) genes are located next to CRISPR sequences. The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as those present within plasmids and phages that provides a form of acquired immunity. RNA harboring the spacer sequence helps Cas proteins recognize and cut exogenous DNA. Other RNA-guided Cas proteins cut foreign RNA. CRISPRs are found in approximately 40% of sequenced bacterial genomes and 90% of sequenced archaea. A simple version of the CRISPR/Cas system, CRISPR/Cas9, has been modified to edit genomes. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added. The Cas9-gRNA complex corresponds with the CAS III crRNA complex in the above diagram. CRISPR/Cas genome editing techniques have many potential applications, including medicine and crop seed enhancement. The use of CRISPR/Cas9-gRNA complex for genome editing was the AAAS's choice for breakthrough of the year in 2015. Bioethical concerns have been raised about the prospect of using CRISPR for germline editing. This book is designed to be a state of the art, superb academic reference work and provide an overview of the topic and give the reader a structured knowledge to familiarize yourself with the topic at the most affordable price possible. The accuracy and knowledge is of an international viewpoint as the edited articles represent the inputs of many knowledgeable individuals and some of the most current knowledge on the topic, based on the date of publication.

Protein-protein Interactions

A Molecular Cloning Manual

Author: Erica Golemis

Publisher: CSHL Press

ISBN: 0879697237

Category: Science

Page: 938

View: 6123

Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.

DNA microarrays

a molecular cloning manual

Author: David Bowtell,Joseph Sambrook

Publisher: Cold Spring Harbor Laboratory Pr

ISBN: N.A

Category: Science

Page: 712

View: 8907

This manual, designed to extend and to complement the information in the best-selling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors--all innovators in a fast-moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.

Molecular Cloning

A Laboratory Manual

Author: Joseph Sambrook,David William Russell

Publisher: CSHL Press

ISBN: 9780879695774

Category: Science

Page: 2344

View: 610

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Molecular Biology Problem Solver

A Laboratory Guide

Author: Alan S. Gerstein

Publisher: John Wiley & Sons

ISBN: 0471461032

Category: Science

Page: 596

View: 9200

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as: Have you selected the most appropriate research strategy? Have you identified the issues critical to your successful application of a technique? Are you familiar with the limitations of a given technique? When should common procedural rules of thumb not be applied? What strategies could you apply to resolve a problem? A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include: Working safely with biological samples and radioactive materials DNA and RNA purification PCR Protein and nucleid acid hybridization Prokaryotic and eukaryotic expression systems Properly using and maintaining laboratory equipment

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