Search Results: handbook-of-biochemical-kinetics

Handbook of Biochemical Kinetics

A Guide to Dynamic Processes in the Molecular Life Sciences

Author: Daniel L. Purich,R. Donald Allison

Publisher: Elsevier

ISBN: 9780080521930

Category: Science

Page: 788

View: 8755

Biochemical kinetics refers to the rate at which a reaction takes place. Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material. The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on techniques and their likely limitations and pitfalls, as well as derivations of fundamental rate equations that characterize biochemical processes. Key Features * Over 750 pages devoted to theory and techniques for studying enzymic and metabolic processes * Over 1,500 definitions of kinetic and mechanistic terminology, with key references * Practical advice on experimental design of kinetic experiments * Extended step-by-step methods for deriving rate equations * Over 1,000 enzymes, complete with EC numbers, reactions catalyzed, and references to reviews and/or assay methods * Over 5,000 selected references to kinetic methods appearing in the Methods in Enzymology series * 72-page Wordfinder that allows the reader to search by keywords * Summaries of mechanistic studies on key enzymes and protein systems * Over 250 diagrams, figures, tables, and structures

Biomolecular Kinetics

A Step-by-Step Guide

Author: Clive R. Bagshaw

Publisher: CRC Press

ISBN: 1351646664

Category: Medical

Page: 446

View: 5037

"a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide" –Mark Leake, University of York "destined to become a standard reference.... Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice." –Michael Geeves, University of Kent "covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" –Stephen Halford, University of Bristol This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A.

Chemical and Biochemical Kinetics

Mechanism of Reactions

Author: Gennadiĭ Efremovich Zaikov

Publisher: Nova Publishers

ISBN: 9781590330692

Category: Science

Page: 198

View: 7485

Chemical & Biochemical Kinetics - Mechanism of Reactions

Comprehensive Enzyme Kinetics

Author: Vladimir Leskovac

Publisher: Springer Science & Business Media

ISBN: 0306483904

Category: Science

Page: 438

View: 6896

Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

Handbook of Biosensors and Biosensor Kinetics

Author: Ajit Sadana,Neeti Sadana

Publisher: Elsevier

ISBN: 9780080932859

Category: Science

Page: 536

View: 6436

Biosensors are essential to an ever-expanding range of applications, including healthcare; drug design; detection of biological, chemical, and toxic agents; environmental monitoring; biotechnology; aviation; physics; oceanography; and the protection of civilian and engineering infrastructures. This book, like the previous five books on biosensors by this author (and one by the co-author), addresses the neglected areas of analyte-receptor binding and dissociation kinetics occurring on biosensor surfaces. Topics are covered in a comprehensive fashion, with homogeneous presentation for the benefit of the reader. The contributors address the economic aspects of biosensors and incorporate coverage of biosensor fabrication and nanobiosensors, among other topics. The comments, comparison, and discussion presented provides a better perspective of where the field of biosensors is heading. Serves as a comprehensive resource on biosensor analysis Examines timely topics such as biosensor fabrication and nanobiosensors Covers economic aspects and medical applications (e.g., the role of analytes in controlling diabetes)

Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 9780080489384

Category: Science

Page: 650

View: 5143

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates

Comprehensive Enzyme Kinetics

Author: Vladimir Leskovac

Publisher: Springer Science & Business Media

ISBN: 0306483904

Category: Science

Page: 438

View: 6577

Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

Handbook of Amylases and Related Enzymes

Their Sources, Isolation Methods, Properties and Applications

Author: The Amylase Research Society o

Publisher: Elsevier

ISBN: 1483299392

Category: Science

Page: 284

View: 5547

This handbook, published to mark the 20th anniversary of The Amylase Research Society of Japan, presents a concise account of the properties and applications of amylases and related enzymes. Enzymes are discussed with reference to their source, isolation method, properties, inhibition, kinetics and protein structure. This information is then applied in the description and interpretation of their use in industry. As well as amylases, other enzymes capable of catalyzing reactions with starch and glycogen, and the further conversion of amylase reaction products for industrial applications are discussed. The text is supported by numerous explanatory figures and tables, and each section is fully referenced.

Initial Rate Enzyme Kinetics

Author: H. J. Fromm

Publisher: Springer Science & Business Media

ISBN: 3642809669

Category: Science

Page: 324

View: 9908

Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.

Enzyme Kinetics: Catalysis and Control

A Reference of Theory and Best-Practice Methods

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 0123809258

Category: Science

Page: 920

View: 610

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Enzyme Kinetics and Mechanism

Author: Paul F. Cook,W. W. Cleland

Publisher: Garland Science

ISBN: 1136844279

Category: Medical

Page: 416

View: 4383

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

The Enzyme Reference

A Comprehensive Guidebook to Enzyme Nomenclature, Reactions, and Methods

Author: Daniel L. Purich,R. Donald Allison

Publisher: Elsevier

ISBN: 0080550819

Category: Science

Page: 929

View: 4344

The aim of this work is to provide a fuller spectrum of information in a single source on enzyme-catalyzed reactions than is currently available in any published reference work or as part of any Internet database. The Enzyme Reference: A Comprehensive Guidebook to Enzyme Nomenclature, Reactions, and Methods includes 20,000 review articles and seminal research papers. Additionally, it provides a novel treatment of so-called ATPase and GTPase reactions to account for the noncovalent substratelike and productlike states of molecular motors, elongation factors, transporters, DNA helicases, G-reulatory proteins, and other energases. Includes a compendium of over 6,000 enzyme reactions (including enzyme commission numbers, alternative names, substrates, products, alternative substrates, and properties) Covers over 900 chemical structures of key metabolites and cofactors Index directs readers to the exact pages for over 9,500 enzyme names

Modeling of Chemical Kinetics and Reactor Design

Author: A. Kayode Coker

Publisher: Gulf Professional Publishing

ISBN: 9780884154815

Category: Technology & Engineering

Page: 1096

View: 7117

Selecting the best type of reactor for any particular chemical reaction, taking into consideration safety, hazard analysis, scale-up, and many other factors is essential to any industrial problem. An understanding of chemical reaction kinetics and the design of chemical reactors is key to the success of the of the chemist and the chemical engineer in such an endeavor. This valuable reference volume conveys a basic understanding of chemical reactor design methodologies, incorporating control, hazard analysis, and other topics not covered in similar texts. In addition to covering fluid mixing, the treatment of wastewater, and chemical reactor modeling, the author includes sections on safety in chemical reaction and scale-up, two topics that are often neglected or overlooked. As a real-world introduction to the modeling of chemical kinetics and reactor design, the author includes a case study on ammonia synthesis that is integrated throughout the text. The text also features an accompanying CD, which contains computer programs developed to solve modeling problems using numerical methods. Students, chemists, technologists, and chemical engineers will all benefit from this comprehensive volume. Shows readers how to select the best reactor design, hazard analysis, and safety in design methodology Features computer programs developed to solve modeling problems using numerical methods

The Inhibitor Index

A Desk Reference on Enzyme Inhibitors, Receptor Antagonists, Drugs, Toxins, Poisons, Biologics, and Therapeutic Leads

Author: Daniel Purich

Publisher: CRC Press

ISBN: 1351730673

Category: Medical

Page: 1362

View: 2069

Metabolic inhibitors and receptor antagonists are indispensable tools for the molecular life scientist. By blocking specific enzymes or receptor-mediated signal transduction cascades, they simplify the analysis of complex cellular processes especially when it is essential to demonstrate that a process of interest is functionally linked to a particular enzyme or receptor. From antibiotics to statins, modern medicine relies on the reliability and ease-of-use of enzyme- and receptor-directed inhibitors and antagonists.The Inhibitor Index is a comprehensive, curated compendium of over 7,800 enzyme inhibitors and receptor antagonists, including many toxins, poisons, and metabolic uncouplers.

Handbook of Electrochemistry

Author: Cynthia G. Zoski

Publisher: Elsevier

ISBN: 0444519580

Category: Science

Page: 892

View: 1369

Electrochemistry plays a key role in a broad range of research and applied areas including the exploration of new inorganic and organic compounds, biochemical and biological systems, corrosion, energy applications involving fuel cells and solar cells, and nanoscale investigations. The Handbook of Electrochemistry serves as a source of electrochemical information, providing details of experimental considerations, representative calculations, and illustrations of the possibilities available in electrochemical experimentation. The book is divided into five parts: Fundamentals, Laboratory Practical, Techniques, Applications, and Data. The first section covers the fundamentals of electrochemistry which are essential for everyone working in the field, presenting an overview of electrochemical conventions, terminology, fundamental equations, and electrochemical cells, experiments, literature, textbooks, and specialized books. Part 2 focuses on the different laboratory aspects of electrochemistry which is followed by a review of the various electrochemical techniques ranging from classical experiments to scanning electrochemical microscopy, electrogenerated chemiluminesence and spectroelectrochemistry. Applications of electrochemistry include electrode kinetic determinations, unique aspects of metal deposition, and electrochemistry in small places and at novel interfaces and these are detailed in Part 4. The remaining three chapters provide useful electrochemical data and information involving electrode potentials, diffusion coefficients, and methods used in measuring liquid junction potentials. * serves as a source of electrochemical information * includes useful electrochemical data and information involving electrode potentials, diffusion coefficients, and methods used in measuring liquid junction potentials * reviews electrochemical techniques (incl. scanning electrochemical microscopy, electrogenerated chemiluminesence and spectroelectrochemistry)

Kinetics of Enzyme-Modifier Interactions

Selected Topics in the Theory and Diagnosis of Inhibition and Activation Mechanisms

Author: Antonio Baici

Publisher: Springer

ISBN: 3709114020

Category: Science

Page: 489

View: 6873

The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.

Handbook of Neurochemistry and Molecular Neurobiology

Practical Neurochemistry Methods

Author: Glen Baker,Susan Dunn,Andrew Holt

Publisher: Springer Science & Business Media

ISBN: 0387303596

Category: Medical

Page: 485

View: 7692

The Handbook is intended to be a service to the neuroscience community, to help in finding available and useful information, to point out gaps in our knowledge, and to encourage continued studies. It represents the valuable contributions of the many authors of the chapters and the guidance of the editors and most important, it represents support for research in this discipline. Based on the rapid advances in the years since the second edition

Enzyme Kinetics

The Steady-State Approach

Author: Paul C. Engel

Publisher: Springer

ISBN: 148993278X

Category: Science

Page: 96

View: 6788

The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.

Handbook of Synthetic Substrates

Author: H.C. Hemker

Publisher: Springer Science & Business Media

ISBN: 9400966903

Category: Medical

Page: 192

View: 3178

The need for a handbook on the use of synthetic substrates for assay of proteases of the coagulation and fibrinolytic systems became evident several years ago during the activities of the Subcommittee on Synthetic Substrates of the International Committee on Thrombosis and Haemo stasis (lCTH). Production of such a handbook, which was recommended during discussions of the ICTH at its meeting in London in 1979 was made possible by the generous efforts of Professor HC Hemker with the aid of several contributors with particular interests in the use of synthetic substrates in coagulation and fibrinolysis. As current Chairman and Secretary General of the ICTH we would like to express our sincere thanks to Professor Hemker for producing this handbook and look for ward to seeing the benefits of this tremendous effort reflected in the advancement of our understanding of thrombosis and hemostasis and the transfer of such knowledge into improved diagnosis and treatment of thrombotic and hemorrhagic disorders. Craig M Jackson Professor of Biological Chemistry Washington University School of Medicine, St. Louis, MO Chairman, ICTH Harold R Roberts Professor of Internal Medicine University of North Carolina, Chapel Hill, NC Secretary General, ICTH ix Foreword The advent of synthetic substrates for the study of blood coagulation and fibrinolysis was a significant step forward in the investigation of these systems. Both basic research and clinical laboratory investigations can profit from these advanced tools.

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