A Step-by-step Handbook for Biologists
Author: Martina Bremer,Rebecca W. Doerge
Statistics at the Bench is a convenient bench-side companion for biologists, designed as a handy reference guide for elementary and intermediate statistical analyses. The expectations for biologists to have a more complete understanding of statistics are growing rapidly. New technologies and new areas of science, such as microarrays, next-generation sequencing, and proteomics, have dramatically increased the need for quantitative reasoning among biologists when designing experiments and interpreting results. Even the most routine informatics tools rely on statistical assumptions and methods that need to be appreciated if the scientific results are to be correct, understood, and exploited fully. This book is not a textbook. It is an essential handbook for working scientists. Statistics at the Bench provides a simple refresher for those who have forgotten what they once knew, and an overview for those wishing to use more quantitative reasoning in their research. Statistical methods, as well as guidelines for the interpretation of results, are explained using simple examples. Throughout the book, examples are accompanied by detailed Excel commands for easy reference.
A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
Author: Dany Spencer Adams
Publisher: CSHL Press
Work at the biology bench requires an ever-increasing knowledge of mathematical methods and formulae. In Lab Math,Dany Spencer Adams has compiled the most common mathematical concepts and methods in molecular biology, and provided clear, straightforward guidance on their application to research investigations. Subjects range from basics such as scientific notation and measuring and making solutions, to more complex activities like quantifying and designing nucleic acids and analyzing protein activity. Tips on how to present mathematical data and statistical analysis are included. A reference section features useful tables, conversion charts and “plug and chug” equations for experimental procedures. This volume is an excellent, structured source of information that in many laboratories is often scattered and informally organized.
A Laboratory Navigator
Author: Kathy Barker
Publisher: CSHL Press
A clue hidden in a toy ship leads Tintin on a dangerous treasure hunt.
A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench
Author: Albert S. Mellick
Publisher: CSHL Press
"The first Lab Ref volume compiled recipes and reference data drawn from a selection of our manuals and was intended to save time and spare frustration." ... "In the same spirit, Lab Ref 2 again assembles in one place a new selection of reference information that should maximize the volume's value in a crowded laboratory environment."--Note.
Author: David J. Glass
Publisher: CSHL Press
Experimental Design for Biologists explains how to establish the framework for an experimental project, including the effects of using a hypothesis–driven approach versus a question/answer approach, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to the negative control, the positive control, and other categories of controls which are perhaps less recognized, such as “assumption controls”, and “experimentalist controls.” Further, there are sections on establishing the experimental system, which includes performing critical “system controls”. While the book does reference the use of statistics, statistics is not the focus of this book, but rather the way the scientist should go about framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. There is often very little formal training in this area for biologists; therefore this text serves as an essential teaching tool for understanding the theory and practice of designing a research plan.
Author: James B. Grace
Publisher: Cambridge University Press
This book, first published in 2006, presents an introduction to the methodology of structural equation modeling, illustrates its use, and goes on to argue that it has revolutionary implications for the study of natural systems. A major theme of this book is that we have, up to this point, attempted to study systems primarily using methods (such as the univariate model) that were designed only for considering individual processes. Understanding systems requires the capacity to examine simultaneous influences and responses. Structural equation modeling (SEM) has such capabilities. It also possesses many other traits that add strength to its utility as a means of making scientific progress. In light of the capabilities of SEM, it can be argued that much of ecological theory is currently locked in an immature state that impairs its relevance. It is further argued that the principles of SEM are capable of leading to the development and evaluation of multivariate theories of the sort vitally needed for the conservation of natural systems.
Methods and Protocols
Author: Michael R. Barnes,Gerome Breen
Publisher: Humana Press
“Your genome is an email attachment” What a difference a few years can make? In 2001, to a global fanfare, the completion of the frst draft sequence of the human genome was announced. This had been a Herculean effort, involving thousands of researchers and millions of dollars. Today, a project to re-sequence 1,000 genomes is well underway, and within a year or two, your own “personal genome” is likely to be available for a few thousand pounds, a price that will undoubtedly decrease further. We are fast approaching the day when your genome will be available as an email attachment (about 4 Mb). The key to this feat is the fact that any two human genomes are more than 99% identical, so rather than representing every base, there is really only a requirement to store the 1% of variable sequence judged against a common reference genome. This brings us directly to the focus of this edition of Methods in Molecular Biology, Genetic Variation. The human genome was once the focus of biology, but now individual genome var- tion is taking the center stage. This new focus on individual variation ultimately democ- tizes biology, offering individuals insight into their own phenotype. But these advances also raise huge concerns of data misuse, misinterpretation, and misunderstanding. The immediacy of individual genomes also serves to highlight our relative ignorance of human genetic variation, underlining the need for more studies of the nature and impact of genetic variation on human phenotypes.
Author: Torsten Hothorn,Brian S. Everitt
Publisher: CRC Press
Like the best-selling first two editions, A Handbook of Statistical Analyses using R, Third Edition provides an up-to-date guide to data analysis using the R system for statistical computing. The book explains how to conduct a range of statistical analyses, from simple inference to recursive partitioning to cluster analysis. New to the Third Edition Three new chapters on quantile regression, missing values, and Bayesian inference Extra material in the logistic regression chapter that describes a regression model for ordered categorical response variables Additional exercises More detailed explanations of R code New section in each chapter summarizing the results of the analyses Updated version of the HSAUR package (HSAUR3), which includes some slides that can be used in introductory statistics courses Whether you’re a data analyst, scientist, or student, this handbook shows you how to easily use R to effectively evaluate your data. With numerous real-world examples, it emphasizes the practical application and interpretation of results.
My Journey of Success, Failure, and Overcoming the Odds
Author: Nick Foles
Category: Biography & Autobiography
*New York Times Bestseller* Get ready to defy the odds when everyone’s counting you out. When the Philadelphia Eagles’ starting quarterback went down with a torn ACL in week 14 of the 2017 NFL season, many fans—and commentators—assumed the Eagles’ season was over. Instead, Nick Foles came off the bench and, against all odds, led the Eagles to their first Super Bowl victory in history. How did Nick get it done—winning MVP honors, silencing the critics, and shocking the world? How did the man who was on the verge of retiring just two seasons earlier stay optimistic and rally the team to an astounding win? How did he stay ready despite numerous trades and discouraging injuries, able to step up in the moment and perform at the top of his game? Believe It offers a behind-the-scenes look at Nick’s unlikely path to the Super Bowl, the obstacles that threatened to hold him back, his rediscovery of his love for the game, and the faith that grounded him through it all. Learn from the way Nick handled the trials and tribulations that made him into the man he is today—and discover a path to your own success.
Author: Eric E. Poehler
Publisher: Oxford University Press
The Traffic Systems of Pompeii is the first sustained examination of the development of road infrastructure in Pompeii--from the archaic age to the eruption of Mt. Vesuvius in 79 CE--and its implications for urbanism in the Roman empire. Eric E. Poehler, an authority on Pompeii's uniquely preserved urban structure, distills over five hundred instances of street-level "wear and tear" to reveal for the first time the rules of the ancient road. From his analysis of curbstones, cobbled surfaces, and ruts emerge the intricacies of the Pompeian traffic system and the changes to its operation over time. Though archaeological expertise forms the backbone of this book, its findings have equally important historical and architectural implications. Later chapters probe the impact of design and infrastructure on social roles and hierarchies among property owners in Pompeii, illuminating the economic forces that push and pull upon the shape of urban space. The final chapters set the road system into its broader context as one major infrastructural and administrative artifact of the Roman empire's deeply urban culture. Where does Pompeii's system fit within the history of Roman traffic control? Is it unique for its innovation, or only for the preservation that permitted its discovery? Poehler marshals evidence from across the Roman world to examine these questions. His measured and thoroughly researched answers make this study a critical step forward in our understanding of infrastructure in the ancient world.
Author: Robert Goldbort
Publisher: Yale University Press
Category: Language Arts & Disciplines
This book encompasses the entire range of writing skills that today's experimental scientist may need to employ. Chapters cover routine forms, such as laboratory notes, abstracts, and memoranda; dissertations; journal articles; and grant proposals. Robert Goldbort discusses how best to approach various writing tasks as well as how to deal with the everyday complexities that may get in the way of ideal practice--difficult collaborators, experiments gone wrong, funding rejections. He underscores the importance of an ethical approach to science and scientific communication and insists on the necessity of full disclosure.
Author: Douglas B. Murphy,Michael W. Davidson
Publisher: John Wiley & Sons
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Author: David Rickwood,J. Robin Harris
As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology.
How Self-Control Works, Why It Matters, and What You Can Do to Get More of It
Author: Kelly McGonigal
Based on Stanford University psychologist Kelly McGonigal's wildly popular course "The Science of Willpower," The Willpower Instinct is the first book to explain the new science of self-control and how it can be harnessed to improve our health, happiness, and productivity. Informed by the latest research and combining cutting-edge insights from psychology, economics, neuroscience, and medicine, The Willpower Instinct explains exactly what willpower is, how it works, and why it matters. For example, readers will learn: Willpower is a mind-body response, not a virtue. It is a biological function that can be improved through mindfulness, exercise, nutrition, and sleep. Willpower is not an unlimited resource. Too much self-control can actually be bad for your health. Temptation and stress hijack the brain's systems of self-control, but the brain can be trained for greater willpower Guilt and shame over your setbacks lead to giving in again, but self-forgiveness and self-compassion boost self-control. Giving up control is sometimes the only way to gain self-control. Willpower failures are contagious—you can catch the desire to overspend or overeat from your friends—but you can also catch self-control from the right role models. In the groundbreaking tradition of Getting Things Done, The Willpower Instinct combines life-changing prescriptive advice and complementary exercises to help readers with goals ranging from losing weight to more patient parenting, less procrastination, better health, and greater productivity at work.
Concepts and Practice for the Future of Biomedical Research
Author: Yoram Vodovotz,Gary An
Are we satisfied with the rate of drug development? Are we happy with the drugs that come to market? Are we getting our money’s worth in spending for basic biomedical research? In Translational Systems Biology, Drs. Yoram Vodovotz and Gary An address these questions by providing a foundational description the barriers facing biomedical research today and the immediate future, and how these barriers could be overcome through the adoption of a robust and scalable approach that will form the underpinning of biomedical research for the future. By using a combination of essays providing the intellectual basis of the Translational Dilemma and reports of examples in the study of inflammation, the content of Translational Systems Biology will remain relevant as technology and knowledge advances bring broad translational applicability to other diseases. Translational systems biology is an integrated, multi-scale, evidence-based approach that combines laboratory, clinical and computational methods with an explicit goal of developing effective means of control of biological processes for improving human health and rapid clinical application. This comprehensive approach to date has been utilized for in silico studies of sepsis, trauma, hemorrhage, and traumatic brain injury, acute liver failure, wound healing, and inflammation. Provides an explicit, reasoned, and systematic approach to dealing with the challenges of translational science across disciplines Establishes the case for including computational modeling at all stages of biomedical research and healthcare delivery, from early pre-clinical studies to long-term care, by clearly delineating efficiency and costs saving important to business investment Guides readers on how to communicate across domains and disciplines, particularly between biologists and computational researchers, to effectively develop multi- and trans-disciplinary research teams
Author: Clifford Geertz
Publisher: Basic Books
Category: Social Science
In The Interpretation of Cultures, the most original anthropologist of his generation moved far beyond the traditional confines of his discipline to develop an important new concept of culture. This groundbreaking book, winner of the 1974 Sorokin Award of the American Sociological Association, helped define for an entire generation of anthropologists what their field is ultimately about.
Author: Institute of Medicine,Roundtable on Evidence-Based Medicine
Publisher: National Academies Press
As our nation enters a new era of medical science that offers the real prospect of personalized health care, we will be confronted by an increasingly complex array of health care options and decisions. The Learning Healthcare System considers how health care is structured to develop and to apply evidence--from health profession training and infrastructure development to advances in research methodology, patient engagement, payment schemes, and measurement--and highlights opportunities for the creation of a sustainable learning health care system that gets the right care to people when they need it and then captures the results for improvement. This book will be of primary interest to hospital and insurance industry administrators, health care providers, those who train and educate health workers, researchers, and policymakers. The Learning Healthcare System is the first in a series that will focus on issues important to improving the development and application of evidence in health care decision making. The Roundtable on Evidence-Based Medicine serves as a neutral venue for cooperative work among key stakeholders on several dimensions: to help transform the availability and use of the best evidence for the collaborative health care choices of each patient and provider; to drive the process of discovery as a natural outgrowth of patient care; and, ultimately, to ensure innovation, quality, safety, and value in health care.
A Laboratory Manual
Author: Donald Charles Rio
Almost all molecular and cellular biology laboratories now handle RNA and this manual is an authoritative source of information and protocols for this purpose, from the basic to the advanced. Required reading for every research laboratory in the life sciences.
Author: Lauren Sompayrac
Publisher: Jones & Bartlett Learning
Category: Family & Relationships
Lauren Sompayrac explains the basics of cancer: what it is, what it does, and what is being done to try to cure it.
An Introduction to Scientific Computing in MATLAB
Author: Pascal Wallisch,Michael E. Lusignan,Marc D. Benayoun,Tanya I. Baker,Adam Seth Dickey,Nicholas G. Hatsopoulos
Publisher: Academic Press
MATLAB for Neuroscientists serves as the only complete study manual and teaching resource for MATLAB, the globally accepted standard for scientific computing, in the neurosciences and psychology. This unique introduction can be used to learn the entire empirical and experimental process (including stimulus generation, experimental control, data collection, data analysis, modeling, and more), and the 2nd Edition continues to ensure that a wide variety of computational problems can be addressed in a single programming environment. This updated edition features additional material on the creation of visual stimuli, advanced psychophysics, analysis of LFP data, choice probabilities, synchrony, and advanced spectral analysis. Users at a variety of levels—advanced undergraduates, beginning graduate students, and researchers looking to modernize their skills—will learn to design and implement their own analytical tools, and gain the fluency required to meet the computational needs of neuroscience practitioners. The first complete volume on MATLAB focusing on neuroscience and psychology applications Problem-based approach with many examples from neuroscience and cognitive psychology using real data Illustrated in full color throughout Careful tutorial approach, by authors who are award-winning educators with strong teaching experience